Technology background
Why cell counting
& viability matter?
Cell counting and viability analysis are fundamental techniques in biological research, drug discovery and bioprocess control. Accurate measurement of cell concentration and health is essential for evaluating culture conditions, monitoring growth and standardizing experiments or manufacturing processes. Traditional methods often rely on dyes, manual interpretation or time-consuming workflows but modern labs and automated systems require faster, more objective and scalable solutions.
our approach
Label-free results through digital holography and AI
Our technology combines digital holographic microscopy with AI-powered image analysis to deliver staining-free cell counting and viability measurements. Instead of using reagents like trypan blue, our platform analyzes intracellular contrast and morphology to assess viability, preserving the sample and simplifying workflows.
Label free analysis without staining or dyes
AI based classification of live and dead cells
High throughput imaging and reconstruction with DHM
Comprehensive viability results with total counts and size distribution
How does it work
A closer look at the
measurement process
1. Illumination
A coherent LED or laser source illuminates the sample. Variations in refractive index scatter the light. The scattered light interferes with the reference wave, forming an interference pattern known as a hologram.
2. Hologram
Advanced algorithms reconstruct the hologram into a sharp, high-contrast image containing quantitative phase and morphological information.
3. Reconstruction
Autofocus functions identify the optimal focal plane for each element, generating a detailed 3D representation of the sample.
4. Classification
AI-driven software analyzes each object such as cells or particles, identifying and classifying them based on specific criteria adaptable to various diagnostic and research applications.
Technology comparison
Advantages of digital holographic microscopy
| Digital holographic microscopy | Conventional brightfield microscopy |
|---|---|
| No sample staining or labeling needed | Often requires staining, labeling or fixation, which can stress and kill cells |
| Generates quantitative phase-based 3D reconstructions | Typically 2D or requires confocal setups for 3D |
| Cells remain intact and usable post-analysis | Sample may be altered or destroyed |
Applications & integration
Flexible use cases. Ready for your platform.
Our cell analysis technology is already commercially available in the fluidlab R-300, where it enables fully digital, label-free cell counting and viability analysis. All in a compact benchtop device used in research and cell culture labs. The same DHM-based module is available for integration into your own analytical or automation systems.
Morphology-Based Viability Classification: Green – live, Blue – dead, Grey – excluded
Suitable for applications such as:
- Cell growth monitoring in bioreactors
- Label-free viability screening in cell line development
- Cell density tracking in microfluidic systems
- Real-time viability checks in automated assays
Designed for integration into:
- Lab automation platforms
- Bioprocess control units
- Portable research analyzers
- Custom assay or cell culture systems
Get expert
support
For technical documentation, OEM integration details or evaluation support, our applications team is here to help.
Head of Analyzer and Applications